Review




Structured Review

Procell Inc siha cl-0210
Siha Cl 0210, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/siha+cl-0210/siha+cells/pm39586885-28-7-12
Average 90 stars, based on 1 article reviews
siha cl-0210 - by Bioz Stars, 2026-09
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Related Articles

Cell Culture:

Article Title: TBP activates DCBLD1 transcription to promote cell cycle progression in cervical cancer.
Article Snippet: of high-risk HPV increases from 12% in normal cytology cases to 89% in CC cases (So et al. 2019).. Regrettably, the majority of patients with CC exhibit limited symptoms during the initial stages, leading to diagnosis at an advanced stage, which is often associated with an unfavorable prognosis and elevated mortality rates (Xin et al. 2022).. Given these insights, researchers and clinicians are facing ongoing challenges in exploring innovative and efficacious treatment approaches.

Article Title: RGS1 and related genes as potential targets for immunotherapy in cervical cancer: computational biology and experimental validation
Article Snippet: HeLa (CL-0101, Procell, China) and SiHa (CL-0210, Procell, China) were cultured in RPMI 1640 (SH30255.01, HyClone, USA) with 10% fetal bovine serum (FBS) (164,210, Procell, China) at 37 °C and 5% CO 2 .

Knockdown:

Article Title: TBP activates DCBLD1 transcription to promote cell cycle progression in cervical cancer.
Article Snippet: of high-risk HPV increases from 12% in normal cytology cases to 89% in CC cases (So et al. 2019).. Regrettably, the majority of patients with CC exhibit limited symptoms during the initial stages, leading to diagnosis at an advanced stage, which is often associated with an unfavorable prognosis and elevated mortality rates (Xin et al. 2022).. Given these insights, researchers and clinicians are facing ongoing challenges in exploring innovative and efficacious treatment approaches.

Article Title: RGS1 and related genes as potential targets for immunotherapy in cervical cancer: computational biology and experimental validation
Article Snippet: HeLa (CL-0101, Procell, China) and SiHa (CL-0210, Procell, China) were cultured in RPMI 1640 (SH30255.01, HyClone, USA) with 10% fetal bovine serum (FBS) (164,210, Procell, China) at 37 °C and 5% CO 2 .

Quantitative RT-PCR:

Article Title: TBP activates DCBLD1 transcription to promote cell cycle progression in cervical cancer.
Article Snippet: of high-risk HPV increases from 12% in normal cytology cases to 89% in CC cases (So et al. 2019).. Regrettably, the majority of patients with CC exhibit limited symptoms during the initial stages, leading to diagnosis at an advanced stage, which is often associated with an unfavorable prognosis and elevated mortality rates (Xin et al. 2022).. Given these insights, researchers and clinicians are facing ongoing challenges in exploring innovative and efficacious treatment approaches.

Article Title: RGS1 and related genes as potential targets for immunotherapy in cervical cancer: computational biology and experimental validation
Article Snippet: HeLa (CL-0101, Procell, China) and SiHa (CL-0210, Procell, China) were cultured in RPMI 1640 (SH30255.01, HyClone, USA) with 10% fetal bovine serum (FBS) (164,210, Procell, China) at 37 °C and 5% CO 2 .

Expressing:

Article Title: TBP activates DCBLD1 transcription to promote cell cycle progression in cervical cancer.
Article Snippet: of high-risk HPV increases from 12% in normal cytology cases to 89% in CC cases (So et al. 2019).. Regrettably, the majority of patients with CC exhibit limited symptoms during the initial stages, leading to diagnosis at an advanced stage, which is often associated with an unfavorable prognosis and elevated mortality rates (Xin et al. 2022).. Given these insights, researchers and clinicians are facing ongoing challenges in exploring innovative and efficacious treatment approaches.

Article Title: RGS1 and related genes as potential targets for immunotherapy in cervical cancer: computational biology and experimental validation
Article Snippet: HeLa (CL-0101, Procell, China) and SiHa (CL-0210, Procell, China) were cultured in RPMI 1640 (SH30255.01, HyClone, USA) with 10% fetal bovine serum (FBS) (164,210, Procell, China) at 37 °C and 5% CO 2 .

Western Blot:

Article Title: TBP activates DCBLD1 transcription to promote cell cycle progression in cervical cancer.
Article Snippet: of high-risk HPV increases from 12% in normal cytology cases to 89% in CC cases (So et al. 2019).. Regrettably, the majority of patients with CC exhibit limited symptoms during the initial stages, leading to diagnosis at an advanced stage, which is often associated with an unfavorable prognosis and elevated mortality rates (Xin et al. 2022).. Given these insights, researchers and clinicians are facing ongoing challenges in exploring innovative and efficacious treatment approaches.

Article Title: RGS1 and related genes as potential targets for immunotherapy in cervical cancer: computational biology and experimental validation
Article Snippet: HeLa (CL-0101, Procell, China) and SiHa (CL-0210, Procell, China) were cultured in RPMI 1640 (SH30255.01, HyClone, USA) with 10% fetal bovine serum (FBS) (164,210, Procell, China) at 37 °C and 5% CO 2 .

Migration:

Article Title: TBP activates DCBLD1 transcription to promote cell cycle progression in cervical cancer.
Article Snippet: of high-risk HPV increases from 12% in normal cytology cases to 89% in CC cases (So et al. 2019).. Regrettably, the majority of patients with CC exhibit limited symptoms during the initial stages, leading to diagnosis at an advanced stage, which is often associated with an unfavorable prognosis and elevated mortality rates (Xin et al. 2022).. Given these insights, researchers and clinicians are facing ongoing challenges in exploring innovative and efficacious treatment approaches.

Article Title: RGS1 and related genes as potential targets for immunotherapy in cervical cancer: computational biology and experimental validation
Article Snippet: HeLa (CL-0101, Procell, China) and SiHa (CL-0210, Procell, China) were cultured in RPMI 1640 (SH30255.01, HyClone, USA) with 10% fetal bovine serum (FBS) (164,210, Procell, China) at 37 °C and 5% CO 2 .

Immunofluorescence:

Article Title: TBP activates DCBLD1 transcription to promote cell cycle progression in cervical cancer.
Article Snippet: of high-risk HPV increases from 12% in normal cytology cases to 89% in CC cases (So et al. 2019).. Regrettably, the majority of patients with CC exhibit limited symptoms during the initial stages, leading to diagnosis at an advanced stage, which is often associated with an unfavorable prognosis and elevated mortality rates (Xin et al. 2022).. Given these insights, researchers and clinicians are facing ongoing challenges in exploring innovative and efficacious treatment approaches.

Article Title: RGS1 and related genes as potential targets for immunotherapy in cervical cancer: computational biology and experimental validation
Article Snippet: HeLa (CL-0101, Procell, China) and SiHa (CL-0210, Procell, China) were cultured in RPMI 1640 (SH30255.01, HyClone, USA) with 10% fetal bovine serum (FBS) (164,210, Procell, China) at 37 °C and 5% CO 2 .



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Image Search Results


Metformin, an AMPK agonist, exerts cytotoxic and anti-tumor effects on cervical cancer. (a) The cell viability of HeLa and SiHa cells following metformin treatment was determined by CCK-8 assay. Data are means ± SD ( n = 3). (b) Western blot analysis was performed to assess PCNA protein levels in cervical cancer cell lines HeLa and SiHa following metformin treatment (left). A statistical analysis of the expression of PCNA (right). Data represent means ± SD ( n = 3). P values were calculated by one-way ANOVA. * P < 0.05, ** P < 0.01, ns, not significant. (c) SiHa cell xenograft tumors from various groups, saline control group, 2 mg/kg cisplatin (CIS), 5 mg/kg metformin (MET), and 50 mg/kg metformin (MET). (d) Xenograft tumor growth curves of SiHa cells from different treatment groups. Tumor volumes formed in BALB/c nude mice were measured every 3 days after implantation of SiHa cells. Data represent means ± SD ( n = 6). P values were calculated by multiple t -test. * P < 0.05, ** P < 0.01, *** P < 0.001. (e) Body weight change (%) curves of mice in different treatment groups. The curves were plotted by collecting mouse weight data every 3 days after the implantation of SiHa cells ( n = 6)

Journal: Cell Communication and Signaling : CCS

Article Title: Activation of AMPK inhibits cervical cancer growth by hyperacetylation of H3K9 through PCAF

doi: 10.1186/s12964-024-01687-7

Figure Lengend Snippet: Metformin, an AMPK agonist, exerts cytotoxic and anti-tumor effects on cervical cancer. (a) The cell viability of HeLa and SiHa cells following metformin treatment was determined by CCK-8 assay. Data are means ± SD ( n = 3). (b) Western blot analysis was performed to assess PCNA protein levels in cervical cancer cell lines HeLa and SiHa following metformin treatment (left). A statistical analysis of the expression of PCNA (right). Data represent means ± SD ( n = 3). P values were calculated by one-way ANOVA. * P < 0.05, ** P < 0.01, ns, not significant. (c) SiHa cell xenograft tumors from various groups, saline control group, 2 mg/kg cisplatin (CIS), 5 mg/kg metformin (MET), and 50 mg/kg metformin (MET). (d) Xenograft tumor growth curves of SiHa cells from different treatment groups. Tumor volumes formed in BALB/c nude mice were measured every 3 days after implantation of SiHa cells. Data represent means ± SD ( n = 6). P values were calculated by multiple t -test. * P < 0.05, ** P < 0.01, *** P < 0.001. (e) Body weight change (%) curves of mice in different treatment groups. The curves were plotted by collecting mouse weight data every 3 days after the implantation of SiHa cells ( n = 6)

Article Snippet: HeLa (HPV18-positive, Cat. NO. CL-0101) and SiHa (HPV16-positive, Cat. NO. CL-0210) obtained from Procell (Wuhan, China), were cultured in DMEM supplemented with 1% penicillin-streptomycin (PS) (Sigma, MO, USA) and 10% FBS (Gibco, NY, USA) under standard conditions (37 °C, 5% CO 2 ).

Techniques: CCK-8 Assay, Western Blot, Expressing, Saline, Control

Effect of AMPK agonist metformin on the AMPK/mTOR pathway. ( a-b ) Representative immunostaining images (left) and their corresponding quantitative results (right) of the intensities of p-AMPK, AMPK, p-mTOR, and mTOR in metformin-treated (0 ~ 20 mM) SiHa (a) and HeLa (b) cells for 48 h. Data represent means ± SD ( n = 3). P values were calculated using a one-way ANOVA test. ns, not significant; # P or * P < 0.05, ## P or ** P < 0.01 (vs. control group). (c-d) Representative immunostaining images (left) and their corresponding quantitative results (right) of the intensities of p-AMPK, AMPK, p-ACC, ACC, p-mTOR, mTOR, p-p70S6K, and p70S6K in SiHa (c) and HeLa (d) cells following treatment with dorsomorphin (10 µM), MHY1485 (10 µM), or metformin (10 mM). Data represent means ± SD ( n = 3). P values were calculated by one-way ANOVA test. ns, not significant; # P or * P < 0.05, ## P or ** P < 0.01

Journal: Cell Communication and Signaling : CCS

Article Title: Activation of AMPK inhibits cervical cancer growth by hyperacetylation of H3K9 through PCAF

doi: 10.1186/s12964-024-01687-7

Figure Lengend Snippet: Effect of AMPK agonist metformin on the AMPK/mTOR pathway. ( a-b ) Representative immunostaining images (left) and their corresponding quantitative results (right) of the intensities of p-AMPK, AMPK, p-mTOR, and mTOR in metformin-treated (0 ~ 20 mM) SiHa (a) and HeLa (b) cells for 48 h. Data represent means ± SD ( n = 3). P values were calculated using a one-way ANOVA test. ns, not significant; # P or * P < 0.05, ## P or ** P < 0.01 (vs. control group). (c-d) Representative immunostaining images (left) and their corresponding quantitative results (right) of the intensities of p-AMPK, AMPK, p-ACC, ACC, p-mTOR, mTOR, p-p70S6K, and p70S6K in SiHa (c) and HeLa (d) cells following treatment with dorsomorphin (10 µM), MHY1485 (10 µM), or metformin (10 mM). Data represent means ± SD ( n = 3). P values were calculated by one-way ANOVA test. ns, not significant; # P or * P < 0.05, ## P or ** P < 0.01

Article Snippet: HeLa (HPV18-positive, Cat. NO. CL-0101) and SiHa (HPV16-positive, Cat. NO. CL-0210) obtained from Procell (Wuhan, China), were cultured in DMEM supplemented with 1% penicillin-streptomycin (PS) (Sigma, MO, USA) and 10% FBS (Gibco, NY, USA) under standard conditions (37 °C, 5% CO 2 ).

Techniques: Immunostaining, Control

Profiling the global lysine acetylation of proteins through AMPK activation. (a-b) Representative Western blot images of the expression levels of acetylated proteins in whole protein extracts of SiHa (a) and HeLa (b) after metformin treatment. (c-d) Representative Western blot images of the expression levels of acetylated proteins in whole histone extracts of SiHa (c) and HeLa (d) after metformin treatment

Journal: Cell Communication and Signaling : CCS

Article Title: Activation of AMPK inhibits cervical cancer growth by hyperacetylation of H3K9 through PCAF

doi: 10.1186/s12964-024-01687-7

Figure Lengend Snippet: Profiling the global lysine acetylation of proteins through AMPK activation. (a-b) Representative Western blot images of the expression levels of acetylated proteins in whole protein extracts of SiHa (a) and HeLa (b) after metformin treatment. (c-d) Representative Western blot images of the expression levels of acetylated proteins in whole histone extracts of SiHa (c) and HeLa (d) after metformin treatment

Article Snippet: HeLa (HPV18-positive, Cat. NO. CL-0101) and SiHa (HPV16-positive, Cat. NO. CL-0210) obtained from Procell (Wuhan, China), were cultured in DMEM supplemented with 1% penicillin-streptomycin (PS) (Sigma, MO, USA) and 10% FBS (Gibco, NY, USA) under standard conditions (37 °C, 5% CO 2 ).

Techniques: Activation Assay, Western Blot, Expressing

AMPK activation induces H3K9 hyperacetylation in vitro. (a) Heatmap analysis of metformin-altered acetylation levels at different lysine sites on histone H3 and H2B in cervical cancer cells based on quantitative acetylproteomic results ( n = 3). (b-c) Representative immunostaining images (left) and quantitative results (right) of the H3K9ac intensity in whole histone extracts of SiHa (b) and HeLa (c) after metformin (0 ~ 20 mM) treatment. Data represent means ± SD ( n = 3). P values were calculated by one-way ANOVA. * P < 0.05, and ** P < 0.01. (d) Identification by LC-MS/MS of H3 4–12 peptides carrying acetylation (ac) at K9. (e-f) Representative immunostaining images (left) and quantitative results (right) of the H3K9ac intensity in SiHa (e) and HeLa (f) after dorsomorphin (10 µM) or (and) MHY1485 (10 µM) or (and) metformin (10 mM) treatment. Data represent means ± SD ( n = 3). P values were calculated by one-way ANOVA. # P or * P < 0.05, ## P or ** P < 0.01

Journal: Cell Communication and Signaling : CCS

Article Title: Activation of AMPK inhibits cervical cancer growth by hyperacetylation of H3K9 through PCAF

doi: 10.1186/s12964-024-01687-7

Figure Lengend Snippet: AMPK activation induces H3K9 hyperacetylation in vitro. (a) Heatmap analysis of metformin-altered acetylation levels at different lysine sites on histone H3 and H2B in cervical cancer cells based on quantitative acetylproteomic results ( n = 3). (b-c) Representative immunostaining images (left) and quantitative results (right) of the H3K9ac intensity in whole histone extracts of SiHa (b) and HeLa (c) after metformin (0 ~ 20 mM) treatment. Data represent means ± SD ( n = 3). P values were calculated by one-way ANOVA. * P < 0.05, and ** P < 0.01. (d) Identification by LC-MS/MS of H3 4–12 peptides carrying acetylation (ac) at K9. (e-f) Representative immunostaining images (left) and quantitative results (right) of the H3K9ac intensity in SiHa (e) and HeLa (f) after dorsomorphin (10 µM) or (and) MHY1485 (10 µM) or (and) metformin (10 mM) treatment. Data represent means ± SD ( n = 3). P values were calculated by one-way ANOVA. # P or * P < 0.05, ## P or ** P < 0.01

Article Snippet: HeLa (HPV18-positive, Cat. NO. CL-0101) and SiHa (HPV16-positive, Cat. NO. CL-0210) obtained from Procell (Wuhan, China), were cultured in DMEM supplemented with 1% penicillin-streptomycin (PS) (Sigma, MO, USA) and 10% FBS (Gibco, NY, USA) under standard conditions (37 °C, 5% CO 2 ).

Techniques: Activation Assay, In Vitro, Immunostaining, Liquid Chromatography with Mass Spectroscopy

PCAF is essential for AMPK-activation-induced H3K9 hyperacetylation. (a-b) Representative immunostaining images (left) and quantitative results (right) of the PCAF and SIRT2 intensity in metformin (0 ~ 20 mM) treated SiHa (a) and HeLa (b) cells. (c) Relative mRNA expression levels of KAT2B, and SIRT2 in SiHa and HeLa after 10 mM metformin treatment ( n = 3). (d) Volcano plots of significantly differentially expressed genes identified in the GEO database between 33 primary cervical cancer tissues and 24 normal cervical epithelial tissues. (e) Dot plots of the relative expression of KAT2B and SIRT2 genes between 33 primary cervical cancer tissues and 24 normal cervical epithelial tissues. (f) Correlation Analysis of AMPK (encoded by PRKAA1) and PCAF (encoded by KAT2B) Expression in the TCGA-CESC Dataset. (g-h) Cell growth curves under different treatments of si-NC, si-PCAF#1, and si-PCAF#2 in SiHa (g) and HeLa (h) cells ( n = 3). ( i-j ) Representative immunostaining images (left) and quantitative results (right) of the PCAF, p300, and H3K9ac intensity in SiHa (i) and HeLa (j) cells after si-PCAF or (and) metformin (10 mM) treatment. Data are means ± SD. The two-tailed Student’s t -test (c) and one-way ANOVA (a/b/i/j) were used to performed comparison between two groups and more groups, respectively. For g/h, P values were calculated by multiple t -test. ns, not significant; # P or * P < 0.05; ## P or ** P < 0.01

Journal: Cell Communication and Signaling : CCS

Article Title: Activation of AMPK inhibits cervical cancer growth by hyperacetylation of H3K9 through PCAF

doi: 10.1186/s12964-024-01687-7

Figure Lengend Snippet: PCAF is essential for AMPK-activation-induced H3K9 hyperacetylation. (a-b) Representative immunostaining images (left) and quantitative results (right) of the PCAF and SIRT2 intensity in metformin (0 ~ 20 mM) treated SiHa (a) and HeLa (b) cells. (c) Relative mRNA expression levels of KAT2B, and SIRT2 in SiHa and HeLa after 10 mM metformin treatment ( n = 3). (d) Volcano plots of significantly differentially expressed genes identified in the GEO database between 33 primary cervical cancer tissues and 24 normal cervical epithelial tissues. (e) Dot plots of the relative expression of KAT2B and SIRT2 genes between 33 primary cervical cancer tissues and 24 normal cervical epithelial tissues. (f) Correlation Analysis of AMPK (encoded by PRKAA1) and PCAF (encoded by KAT2B) Expression in the TCGA-CESC Dataset. (g-h) Cell growth curves under different treatments of si-NC, si-PCAF#1, and si-PCAF#2 in SiHa (g) and HeLa (h) cells ( n = 3). ( i-j ) Representative immunostaining images (left) and quantitative results (right) of the PCAF, p300, and H3K9ac intensity in SiHa (i) and HeLa (j) cells after si-PCAF or (and) metformin (10 mM) treatment. Data are means ± SD. The two-tailed Student’s t -test (c) and one-way ANOVA (a/b/i/j) were used to performed comparison between two groups and more groups, respectively. For g/h, P values were calculated by multiple t -test. ns, not significant; # P or * P < 0.05; ## P or ** P < 0.01

Article Snippet: HeLa (HPV18-positive, Cat. NO. CL-0101) and SiHa (HPV16-positive, Cat. NO. CL-0210) obtained from Procell (Wuhan, China), were cultured in DMEM supplemented with 1% penicillin-streptomycin (PS) (Sigma, MO, USA) and 10% FBS (Gibco, NY, USA) under standard conditions (37 °C, 5% CO 2 ).

Techniques: Activation Assay, Immunostaining, Expressing, Two Tailed Test, Comparison

AMPK/mTOR axis participation in the regulation of PCAF acetylation activity. (a-b) Representative immunostaining images (left) and quantitative results (right) of the p-AMPK, AMPK, p-mTOR, and mTOR intensity in SiHa (a) and HeLa (b) cells after si-PCAF or (and) metformin (10 mM) treatment. (c) Representative immunostaining images (left) and quantitative results (right) of the PCAF intensity in SiHa (up) and HeLa (down) after dorsomorphin (10 µM) or (and) MHY1485 (10 µM) or (and) metformin (10 mM) treatment. Data represent means ± SD. P values were calculated by one-way ANOVA test. ns, not significant; # P or * P < 0.05; ## P or ** P < 0.01

Journal: Cell Communication and Signaling : CCS

Article Title: Activation of AMPK inhibits cervical cancer growth by hyperacetylation of H3K9 through PCAF

doi: 10.1186/s12964-024-01687-7

Figure Lengend Snippet: AMPK/mTOR axis participation in the regulation of PCAF acetylation activity. (a-b) Representative immunostaining images (left) and quantitative results (right) of the p-AMPK, AMPK, p-mTOR, and mTOR intensity in SiHa (a) and HeLa (b) cells after si-PCAF or (and) metformin (10 mM) treatment. (c) Representative immunostaining images (left) and quantitative results (right) of the PCAF intensity in SiHa (up) and HeLa (down) after dorsomorphin (10 µM) or (and) MHY1485 (10 µM) or (and) metformin (10 mM) treatment. Data represent means ± SD. P values were calculated by one-way ANOVA test. ns, not significant; # P or * P < 0.05; ## P or ** P < 0.01

Article Snippet: HeLa (HPV18-positive, Cat. NO. CL-0101) and SiHa (HPV16-positive, Cat. NO. CL-0210) obtained from Procell (Wuhan, China), were cultured in DMEM supplemented with 1% penicillin-streptomycin (PS) (Sigma, MO, USA) and 10% FBS (Gibco, NY, USA) under standard conditions (37 °C, 5% CO 2 ).

Techniques: Activity Assay, Immunostaining